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Mouse ovarian factors with angiogenic activities: partial characterization
1Department of Animal Science, Faculty of Agriculture, Kyoto University, Japan.
Endocrinologia Japonica
|June 1, 1990
Summary
Mouse ovarian extracts promote new blood vessel growth in a dose-dependent manner, with activity enhanced by follicle-stimulating hormone (FSH) and retained after purification.
Area of Science:
- Reproductive Biology
- Angiogenesis Research
- Biochemistry
Background:
- Ovarian extracts contain factors influencing physiological processes.
- Neovascularization, or new blood vessel formation, is crucial for tissue repair and development.
Purpose of the Study:
- To investigate the angiogenic potential of nonluteal mouse ovarian extracts.
- To determine the effect of follicle-stimulating hormone (FSH) on ovarian angiogenic activity.
Main Methods:
- Assay for neovascularization using Elvax film implants in mouse rectus abdominis muscle.
- Treatment of mice with FSH to evaluate its impact on ovarian extract activity.
- Purification of ovarian extracts using Concanavalin A (Con A)-Sepharose 4B affinity chromatography and ammonium sulfate precipitation.
Main Results:
- Ovarian extracts demonstrated dose-dependent neovascularization.
- FSH treatment significantly increased the angiogenic activity of ovarian extracts.
- Angiogenic activity remained stable after heating and charcoal adsorption.
- Purification via Con A-Sepharose and ammonium sulfate precipitation concentrated the angiogenic factors.
Conclusions:
- Nonluteal mouse ovarian extracts possess significant angiogenic properties.
- FSH enhances the production or release of angiogenic factors from the ovary.
- The angiogenic factors are heat-stable and can be purified, suggesting potential therapeutic applications.