Unraveling the spatial distribution of immunoglobulins, enzymes, and polyelectrolytes within layer-by-layer
N F Ferreyra1, E S Forzani, M López Teijelo
1INFIQC-Departamento de Físicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Córdoba, Argentina. ferreyra@mail.fcq.unc.edu.ar
Abstract:
The ellipsometric characterization of a layer-by-layer electrostatically self-assembled multilayer of polyphenol oxidase and alkaline phosphatase with the polycation poly(dimethyldiallylammonium chloride) built on an immunologic layer formed by immunoglobulin G (IgG) and glucose oxidase-conjugated anti-IgG (IgG-GOD) on glassy carbon is reported. The step-by-step evolution of the psi-Delta ellipsometric angles was followed during film growth. Two optical models, named the three-layer film model and reorganization film model, were employed and found suitable for ellipsometric data interpretation. A comparative analysis of film optical properties, film thickness, and ellipsometric mass assessed from both models is also presented.
