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Related Experiment Videos

A dynamical model for phospholipid-calcium binding.

F Lara-Ochoa1

  • 1Centro de Investigacion Sobre Fijacion de Nitrogeno, UNAM Apdo. Postal 565-A, Cuernavaca, Mor., Mexico.

Biophysical Chemistry
|May 1, 1991
PubMed
Summary
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A new model explains how ionic binding causes gel-liquid crystalline transitions in cell membranes. This research highlights how phospholipid domains and membrane heterogeneities influence vesicle fusion, particularly in the presence of calcium ions.

Area of Science:

  • Biophysics
  • Physical Chemistry
  • Materials Science

Background:

  • Cell membranes undergo phase transitions between gel and liquid crystalline states.
  • Ionic binding, particularly with divalent cations like Ca2+, is known to influence membrane properties and function.
  • Understanding these transitions is crucial for cellular processes like membrane fusion and transport.

Purpose of the Study:

  • To propose a theoretical model for the isothermal gel-liquid crystalline transition in membranes induced by ionic binding.
  • To investigate the role of phospholipid-calcium interactions and resulting spatial heterogeneities in membrane behavior.
  • To provide a framework for understanding enhanced vesicle fusion in the presence of Ca2+.

Main Methods:

  • Development of a theoretical model using a Ginzburg-Landau functional to describe long-range order during the transition.

Related Experiment Videos

  • Calculation of chemical potential to derive the mass current of phospholipids.
  • Incorporation of phospholipid-calcium interaction kinetics and flux divergence into the conservation of mass equation.
  • Analysis of a circular membrane model in polar coordinates.

    • Main Results:

    • The model predicts an heterogeneous distribution of phospholipid domains in gel and liquid crystalline phases.
    • Calculations show a mass current of phospholipids in the gel phase described by an order parameter.
    • The model suggests that spatial heterogeneities at domain boundaries can lead to membrane destabilization.

    Conclusions:

    • The proposed model successfully describes the gel-liquid crystalline transition driven by ionic binding.
    • The findings support the hypothesis that membrane heterogeneities arising from such transitions contribute to enhanced vesicle fusion.
    • This work offers a theoretical basis for experimental observations of domain formation and fusion in biological membranes.