Related Experiment Video
Updated: Jul 19, 2026
Production and Testing of Antimicrobial Peptides and Their Mimics
Published on: April 10, 2026
Synthesis of a molecularly imprinted polymer for the selective solid-phase extraction of chloramphenicol from honey
Christina Schirmer1, Hans Meisel
1Institute of Dairy Chemistry and Technology, Federal Research Centre for Nutrition and Food (BfEL) Location Kiel, Hermann-Weigmann-Strasse 1, D-24103 Kiel, Germany.
Abstract:
Solid-phase extraction (SPE) with a molecularly imprinted polymer (MIP) as sorbent has been investigated for the clean-up of the broad-spectrum bacteriostatic antibiotic chloramphenicol (CAP) in honey samples. The MIP was prepared by using methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EDMA) as cross-linker, chloroform as porogen and CAP as template molecule. The binding behaviour of the template CAP on the MIP was evaluated by high-performance liquid chromatography, and then the MIP was applied as a sorbent in SPE to selectively extract CAP from honey. It was shown that recoveries of nearly 100% of a CAP standard solution and up to 94% from spiked honey samples could be obtained after SPE.
