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Estimation of hydroxyl radical generation by salicylate hydroxylation method in multiple organs of mice exposed to
Shunji Ueno1, Takashige Kashimoto, Nobuyuki Susa
1School of Veterinary Medicine and Animal Sciences, Kitasato University, Laboratory of Veterinary Public Health, Higashi 23-35-1, Towada, Japan. ueno@vmas.kitasato-u.ac.jp
Abstract:
Appropriate experimental conditions for the estimation of hydroxyl radical generation by salicylate hydroxylation were determined for multiple organs of X-irradiated mice in vivo. The in vitro experiments showed that there were significant correlations between the salicylic acid (SA) concentration, the amount of 2,3-dihydroxy benzoic acid (2,3-DHBA) and the X-ray exposure dose, and we obtained two linear-regression equations to calculate the amounts of hydroxyl radicals generated by the X-irradiation. The optimum dosage of SA and the appropriate sampling time for in vivo experiments was determined, and significant increases in the ratio of 2,3-DHBA to SA were detected in several organs of mice after X-irradiation. The hydroxyl radical equivalents of the 2,3-DHBA increases were also calculated. Our results clearly demonstrated the usefulness of the salicylate hydroxylation method in estimating hydroxyl radical generation in multiple organs in vivo.
