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Related Experiment Videos

PAX8 expression in human bladder cancer.

Lucia Pellizzari1, Cinzia Puppin, Laura Mariuzzi

  • 1Istituto di Genetica, Policlinico Universitario di Udine, Napoli, Italy.

Oncology Reports
|October 4, 2006
PubMed
Summary
This summary is machine-generated.

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PAX8 (paired-box gene 8) is expressed in non-invasive urothelial tumors but not in normal adult bladders, suggesting its role in bladder cancer development. PAX8 is also found in embryonic bladder tissue.

Area of Science:

  • Urothelial Carcinogenesis
  • Molecular Oncology
  • Developmental Biology

Background:

  • PAX8 (paired-box gene 8) is a crucial transcription factor involved in the development of the urinary tract.
  • Its precise role in adult bladder tissue and urothelial neoplasms remains incompletely understood.

Purpose of the Study:

  • To investigate the expression patterns of PAX8 in normal adult bladder tissue and various non-invasive urothelial tumors.
  • To explore the presence of PAX8 in embryonic bladder and bladder cancer cell lines.

Main Methods:

  • Immunohistochemistry was employed to assess PAX8 protein expression in 30 non-invasive urothelial tumors and normal bladder mucosa.
  • RT-PCR was used to evaluate PAX8 mRNA expression in normal bladder mucosa, tumors, embryonic bladders, and bladder cancer cell lines.

Related Experiment Videos

  • Splicing isoform analysis and the effect of sodium butyrate treatment on cell lines were also investigated.

    • Main Results:

    • PAX8 protein was detected in 28 out of 30 non-invasive urothelial tumors but was absent in normal adult bladder tissue.
    • PAX8 mRNA was expressed in all 13 tested bladder tumors and 2 embryonic bladders, but not in 6 normal adult bladder samples.
    • PAX8 was expressed in all tested bladder cancer cell lines, exhibiting heterogeneous splicing isoforms, partially modulated by sodium butyrate.

    Conclusions:

    • PAX8 expression is significantly associated with non-invasive urothelial tumors and absent in normal adult bladder, indicating a potential role as a biomarker.
    • PAX8 expression in embryonic bladder suggests its involvement in early urinary tract development.
    • The heterogeneity of PAX8 splicing isoforms in tumors and cell lines warrants further investigation into their functional significance.