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Updated: Jul 19, 2026
Rat Mesentery Angiogenesis Assay
Published on: June 18, 2011
Antiangiogenic activity of a concentrated effective microorganism fermentation extract
Chung Hin Chui1, Roberto Gambari, Fung Yi Lau
1Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, PR China.
Abstract:
We have previously demonstrated the possible growth inhibitory activity of both first generation of the effective microorganism fermentation extract (EM-X) as well as the second generation (EM-X2) on cancer cell lines in vitro. The possible anti-angiogenic potential of EM-X has not been reported. Herein we show that using the concentrated EM-X, the growth of human umbilical cord endothelial cells (HUCE) was significantly inhibited in vitro. Enzyme linked immunosorbent assay suggested that the concentrated EM-X is able to reduce the level of vascular endothelial growth factor (VEGF) from Hep3B hepatocellular carcinoma (HCC) cells. The conditioned culture medium obtained from the concentrated EM-X incubated Hep3B HCC cells possessed significant antiproliferative effect on the HUCE cells. Moreover, in vivo chick chorioallantoic membrane assay further demonstrated that the concentrated EM-X is able to greatly inhibit the basic fibroblast growth factor induced angiogenesis from chick embryo experiment. We speculate that the anti-cancer potential of this concentrated EM-X involved growth inhibition on cancer cell and antiangiogenic effect on HUCE cells.
Insights
This study shows concentrated Effective Microorganism-X (EM-X) inhibits human umbilical cord endothelial cell growth and angiogenesis. EM-X may offer anti-cancer potential by impacting cancer cells and blood vessel formation.
Area of Science:
- Biochemistry
- Cell Biology
- Angiogenesis Research
Background:
- Previous studies indicated Effective Microorganism-X (EM-X) and EM-X2 inhibit cancer cell lines in vitro.
- The anti-angiogenic potential of EM-X has not been previously reported.
Purpose of the Study:
- To investigate the anti-angiogenic effects of concentrated EM-X.
- To explore the impact of EM-X on endothelial cell proliferation and vascular endothelial growth factor (VEGF) levels.
Main Methods:
- In vitro inhibition of human umbilical cord endothelial cells (HUCE) growth by concentrated EM-X.
- Enzyme-linked immunosorbent assay (ELISA) to measure VEGF levels in Hep3B hepatocellular carcinoma (HCC) cells.
- In vivo chick chorioallantoic membrane (CAM) assay to assess angiogenesis inhibition.
Main Results:
- Concentrated EM-X significantly inhibited HUCE cell growth in vitro.
- EM-X reduced VEGF levels in Hep3B HCC cells.
- EM-X demonstrated significant inhibition of basic fibroblast growth factor-induced angiogenesis in the chick CAM assay.
Conclusions:
- Concentrated EM-X exhibits anti-angiogenic properties.
- The anti-cancer potential of EM-X may involve direct growth inhibition of cancer cells and anti-angiogenic effects.
