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Related Experiment Video

Updated: Jun 25, 2026

Forced Flowering in Mandarin Trees under Phytotron Conditions

Forced Flowering in Mandarin Trees under Phytotron Conditions

Published on: March 6, 2019

An efficient, in vitro cyclic production of shoots from adult trees of Crataeva nurvala Buch. Ham.

Neetika Walia¹, Amandeep Kaur, Shashi B Babbar

¹Department of Botany, University of Delhi, Delhi 110007, India. neetika.walia@gmail.com

Plant Cell Reports

|October 4, 2006

Summary

This summary is machine-generated.

A new, efficient micropropagation protocol for the medicinal tree Crataeva nurvala has been developed. This scalable, cyclic method can generate over 5400 shoots from a single explant in six months.

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Area of Science:

Plant Biotechnology
Medicinal Botany
Horticulture

Background:

Crataeva nurvala is a valuable medicinal tree.
Efficient propagation methods are crucial for its conservation and utilization.
Previous propagation techniques may lack scalability or efficiency.

Purpose of the Study:

To develop an efficient, scalable, and cyclic micropropagation protocol for Crataeva nurvala.
To optimize media components for shoot proliferation and elongation.
To establish a reliable method for regenerating whole plants from explants.

Main Methods:

Nodal explants from mature Crataeva nurvala trees were cultured on Murashige and Skoog (MS) medium with 6-benzylaminopurine (BAP).
Regenerated shoots were further cultured for shoot proliferation and elongation.
Casein hydrolysate and naphthaleneacetic acid (NAA) were tested for optimizing shoot number and root induction.
Plantlets were acclimatized and transplanted to soil.

Main Results:

A two-step protocol using MS medium with 2.22 microM BAP efficiently produced multiple shoots from nodal explants.
The process was recurrent, utilizing both nodal and leaf explants from regenerated shoots.
Over 5400 shoots were produced from a single explant in six months.
Casein hydrolysate significantly enhanced shoot regeneration, with 100 mg/L being optimal.
Shoots rooted effectively on 1/2 MS medium with 0.11-0.54 microM NAA.
Acclimatized plantlets showed successful soil transplantation.

Conclusions:

An efficient, cyclic, and commercially viable micropropagation protocol for Crataeva nurvala has been established.
The protocol enables large-scale production of genetically identical plantlets from mature trees.
This method supports conservation efforts and the sustainable supply of this medicinal tree.