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Updated: Jul 19, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
Separation of parabens in capillary electrochromatography using poly(styrene-divinylbenzene-methacrylic acid)
Hsi-Ya Huang1, I-Yun Huang, Heng Yi Lin
1R&D Center for Membrane Technology, CYCU, Department of Chemistry, Chung Yuan Christian University, Chung-Li, Taiwan, ROC. hyhuang@cycu.edu.tw
Abstract:
In this study, a series of poly(styrene-divinylbenzene-methacrylic acid) monolithic capillaries was used as the separation column of CEC for the analyses of parabens in commercial pharmaceutical and cosmetic products. The results showed that the chromatographic characteristics of these analytes were strongly affected by the preparation condition of the monolithic column including monomer content, porogenic solvent composition, and polymerization time. Baseline separations were markedly sped up by lowering the polymerization time without any obvious loss of resolution. Furthermore, mobile-phase composition (pH, ACN, and electrolyte concentration) was also able to effectively improve the separation behavior. Similar to the influence of lowering the polymerization time, retention times for all analytes were significantly shortened in the CEC method by decreasing the electrolyte concentration in the mobile phase.
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