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Related Experiment Videos

A mouse monoclonal antibody reactive preferentially with human IgM lambda.

T Ohno1, M D Cooper, H Kubagawa

  • 1Department of Medicine, University of Alabama, Birmingham.

Hybridoma
|October 1, 1990
PubMed
Summary
This summary is machine-generated.

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A novel mouse monoclonal antibody (mAb), LP4, specifically targets IgM lambda B cells and plasma cells. This antibody is a valuable tool for studying B cell differentiation and identifying specific B cell populations.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Monoclonal antibodies (mAbs) are crucial tools in immunological research.
  • Identifying specific cell surface markers aids in understanding B cell development and disease states.

Purpose of the Study:

  • To characterize a unique mouse monoclonal antibody (mAb), LP4, generated against human IgM paraprotein.
  • To determine the specificity and utility of mAb LP4 in identifying distinct B cell populations.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) was used to test mAb reactivity.
  • Immunofluorescence and biosynthetic analysis were employed to identify target cells and molecules.

Main Results:

  • The LP4 mAb reacted exclusively with IgM lambda paraproteins and not IgM kappa.

Related Experiment Videos

  • LP4 identified approximately 30% of peripheral blood IgM+ B cells and 40% of IgM+ plasma cells.
  • Biosynthetic analysis confirmed LP4 recognizes most IgM lambda and a small subset of IgM kappa molecules.

    • Conclusions:

    • The LP4 mAb is a specific marker for IgM lambda B cells and plasma cells.
    • This antibody serves as a valuable tool for analyzing B cell differentiation, particularly pre-B and B cell stages.