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High-performance liquid chromatographic determination of proteins by post-column fluorescence derivatization with
1Central Research Laboratory, SS Pharmaceutical Co. Ltd., Chiba, Japan.
Journal of Chromatography
|September 28, 1990
Abstract:
A post-column derivatization method for the high-performance liquid chromatography of peptides and proteins giving a fluorescence intensity proportional to the number of peptide bonds is described. Peptide bonds were chlorinated with hypochlorite and the N-chlorite formed was allowed to react with thiamine to give fluorescent thiochrome. This method was applied the determination of membrane-forming proteins of microorganisms.