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A microchip sensor for calcium determination.

P Caglar1, S A Tuncel, N Malcik

  • 1Department of Chemistry, Hacettepe University, Beytepe 06532, Ankara, Turkey. percag@hacettepe.edu.tr

Analytical and Bioanalytical Chemistry
|October 5, 2006
PubMed
Summary

A novel microchip sensor effectively determines calcium (Ca2+) ions using immobilized arsenazo III dye on polymer beads. This reusable sensor shows promise for clinical analysis of calcium in serum samples.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biomedical Engineering

Background:

  • Accurate determination of calcium ions (Ca2+) is crucial for clinical diagnostics.
  • Existing methods for Ca2+ detection may require complex sample preparation or lack portability.
  • Development of sensitive and reusable sensors for Ca2+ is an ongoing area of research.

Purpose of the Study:

  • To develop a novel glass-PDMS microchip-based sensor for Ca2+ determination.
  • To immobilize arsenazo III dye onto functionalized polymer beads for enhanced sensing capabilities.
  • To evaluate the sensor's performance, including linear range, limit of detection, and reusability for clinical applications.

Main Methods:

  • Fabrication of a microfluidic sensor device integrating glass and PDMS components.
  • Covalent modification of poly(p-chloromethylstyrene) (PCMS) beads with polyethylenimine (PEI) for dye immobilization.
  • Immobilization of arsenazo III dye onto PEI-modified PCMS beads.
  • Utilizing fiber optic reflectance measurements for Ca2+ detection within the microfluidic channel.
  • Optimization of sensor parameters including pH, voltage, and regeneration protocols.

Main Results:

  • Successfully immobilized a maximum of 373.71 mg g(-1) of arsenazo III onto PEI-attached PCMS beads at pH 1.
  • The microchip sensor demonstrated a linear range of 3.57 x 10(-5) - 5.71 x 10(-4) M Ca2+ at an optimal potential of 0.8 kV and pH 9.0.
  • Achieved a limit of detection (LOD) of 2.68 x 10(-5) M for Ca2+.
  • The sensor exhibited good reusability after regeneration with HCl solution.
  • The developed biosensor was successfully applied for clinical analysis of calcium ions in serum samples.

Conclusions:

  • A novel and reusable microchip-based biosensor for Ca2+ determination has been successfully developed.
  • The sensor utilizes immobilized arsenazo III on functionalized polymer beads and fiber optic reflectance measurements.
  • The sensor exhibits excellent performance characteristics, including a wide linear range and low LOD.
  • The microchip biosensor shows significant potential for accurate and efficient clinical analysis of calcium in biological samples.