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[FTIR characterization of the secondary structure of a staphylokinase variant (K35R) encapsulated within PLGA
Jin-Tian He1, Gai-Zhen Wang, Hou-Yan Song
1College of Life Science, Hebei Normal University, Shijiazhuang 050016, China.
Abstract:
The secondary structure of a staphylokinase variant (K35R, DGR) encapsulated in poly (lactic-co-glycolic acid) (PLGA) microspheres was quantitatively examined by Fourier transform infrared (FTIR) spectroscopy. Resolution enhancement technique and Fourier deconvolution were combined with band with curve-fitting procedures to quantitate the spectral information from the amide I bands. Nine component bands were found under the broad, nearly featureless amide I bands and assigned to alpha-helix, beta-sheet, turn and irregular (random) structures. The changes of bands at 1 651 and 1 623 cm(-1) after encapsulation were discussed.
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