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Related Experiment Videos

Histamine release with a solid-phase-coupled allergen.

H Mita1, K Tadokoro, H Yasueda

  • 1National Sagamihara Hospital, Clinical Research Center for Rheumato-Allergology, Kanagawa, Japan.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|November 5, 1990
PubMed
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Immobilizing mite allergens for histamine release assays showed comparable spontaneous release and reproducibility to soluble allergens. However, immobilized allergens yielded a lower maximal histamine release in human leukocytes.

Area of Science:

  • Immunology
  • Allergen research
  • Cellular assays

Background:

  • Histamine release assays are crucial for diagnosing allergies.
  • Soluble allergens can exhibit dose-dependent inhibition at high concentrations.
  • Immobilization of allergens offers a potential alternative for assay standardization.

Purpose of the Study:

  • To evaluate the efficacy of immobilized mite allergens in histamine release assays.
  • To compare the performance of immobilized allergens with soluble allergens.
  • To investigate the impact of allergen concentration and immobilization matrix on histamine release.

Main Methods:

  • Mite allergen was coupled to Sepharose, paper disks, and microcrystalline cellulose.
  • Histamine release assays were performed using human leukocytes and immobilized or soluble allergens.

Related Experiment Videos

  • Spontaneous release, reproducibility, and dose-response effects were analyzed.

    • Main Results:

    • Immobilized allergen assays demonstrated comparable spontaneous histamine release and reproducibility to soluble allergen assays.
    • Histamine release increased with immobilized allergen concentration up to a maximum, unlike soluble allergens which showed decreased release at high concentrations.
    • Maximal histamine release with immobilized allergens was approximately 20% lower than with soluble allergens, irrespective of immobilization matrix or density.

    Conclusions:

    • Immobilized mite allergens provide a viable alternative for histamine release assays, offering consistent results.
    • The assay format using immobilized allergens overcomes the high-dose inhibition observed with soluble allergens.
    • Further optimization may be needed to match the maximal histamine release levels achieved with soluble allergens.