Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Candida albicans strain maintenance, replacement, and microvariation demonstrated by multilocus sequence typing.

F C Odds1, A D Davidson, M D Jacobsen

  • 1Aberdeen Fungal Group, Institute of Medical Sciences, Aberdeen AB25 2ZD, United Kingdom. f.odds@abdn.ac.uk

Journal of Clinical Microbiology
|October 6, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Laser-induced graphene-based aptasensor for the selective detection of Escherichia coli in urine samples.

Talanta·2024
Same author

Association between body mass and hypotension in dogs under general anaesthesia.

The Journal of small animal practice·2023
Same author

The interacting brain: Dynamic functional connectivity among canonical brain networks dissociates cooperative from competitive social interactions.

NeuroImage·2023
Same author

Midodrine and albumin as a possible "winning pair" in managing paracentesis-induced circulatory dysfunction: a clinical case report.

European review for medical and pharmacological sciences·2023
Same author

Microscopic colitis, a forgotten condition: a clinical case and review of the literature.

European review for medical and pharmacological sciences·2022
Same author

Sweat analysis with a wearable sensing platform based on laser-induced graphene.

APL bioengineering·2022

Multilocus sequence typing (MLST) and ABC typing reveal Candida albicans strain diversity within patients, showing stable carriage and occasional strain replacement. Genetic variation may arise from recombination or ploidy changes.

Area of Science:

  • Medical Mycology
  • Molecular Epidemiology
  • Microbial Genetics

Background:

  • Candida albicans is a significant opportunistic fungal pathogen.
  • Understanding strain diversity is crucial for infection control and treatment.
  • Previous studies suggest C. albicans can be carried stably by individuals.

Purpose of the Study:

  • To investigate the genetic diversity and stability of Candida albicans strains within individual patients.
  • To compare the discriminatory power of multilocus sequence typing (MLST) and rRNA gene ABC typing.
  • To explore mechanisms contributing to genetic variation in C. albicans.

Main Methods:

  • Typing of 165 Candida albicans isolates from 44 sources using MLST and ABC typing.
  • Determination of mating-type-like locus (MTL) homozygosity/heterozygosity.

Related Experiment Videos

  • Analysis of strain diversity within individual patients, including isolates from blood and non-sterile sites.

    • Main Results:

    • MLST and ABC typing demonstrated indistinguishable or highly related strain types within most patients over time and across different body sites.
    • Evidence of strain replacement was observed in one patient across different hospital admissions.
    • Microvariation, often due to loss of heterozygosity, was noted, and some isolates showed intermittent genetic differences suggesting recombination or ploidy changes.

    Conclusions:

    • C. albicans exhibits stable strain carriage within individuals, consistent with existing views.
    • MLST and ABC typing are effective tools for assessing C. albicans strain diversity.
    • A minority of isolates display high genetic diversity, potentially through recombination, gene conversion, or chromosomal ploidy changes.