Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bleomycin effects on mouse meiotic chromosomes.

P Poorman-Allen1, L C Backer, I D Adler

  • 1Wellcome Research Laboratories, Research Triangle Park, NC 27709.

Mutagenesis
|November 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recommended Resting-State fMRI Acquisition and Preprocessing Steps for Preoperative Mapping of Language and Motor and Visual Areas in Adult and Pediatric Patients with Brain Tumors and Epilepsy.

AJNR. American journal of neuroradiology·2024
Same author

Peer Learning Program Metrics: A Pediatric Neuroradiology Example.

AJNR. American journal of neuroradiology·2022
Same author

A multicenter prospective evaluation of owner medication adherence for feline cardiovascular disease in the referral setting.

Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology·2022
Same author

Glutamine Imaging: A New Avenue for Glioma Management.

AJNR. American journal of neuroradiology·2021
Same author

An automated approach to determine antibody endpoint titers for COVID-19 by an enzyme-linked immunosorbent assay.

Immunohematology·2021
Same author

Altered Processing of Complex Visual Stimuli in Patients with Postconcussive Visual Motion Sensitivity.

AJNR. American journal of neuroradiology·2021
Same journal

How genotoxic is the indoor air environment?

Mutagenesis·2026
Same journal

Unveiling the Genotoxic Safety and Antioxidant-Mediated DNA Protective Mechanisms of Triammonium Citrate: An Integrated In Vitro and In Silico Approach.

Mutagenesis·2026
Same journal

Nutritional maternal factors affect the frequency of DNA damage in newborns.

Mutagenesis·2026
Same journal

Potassium bromate as a probable human Carcinogen: multi-organ toxicity and chemopreventive potential of dietary antioxidants-a comprehensive review.

Mutagenesis·2026
Same journal

DNA damage in the leukocytes of nanocomposite-exposed workers.

Mutagenesis·2026
Same journal

Ultra-Processed Foods and Diet Quality in Long COVID: Associations with Symptom Burden, DNA Damage, and Inflammation.

Mutagenesis·2026
See all related articles

Bleomycin (BLM) causes structural damage to mouse meiotic chromosomes, particularly when administered during S phase. While BLM is clastogenic, its effects on meiosis differ from its general activity.

Area of Science:

  • Reproductive toxicology
  • Genetics and heredity
  • Cell biology

Background:

  • Bleomycin (BLM) is a radiomimetic chemical agent known for its DNA-damaging properties.
  • Understanding the effects of genotoxic agents on germ cells is crucial for reproductive health and genetic integrity.
  • Meiosis involves complex chromosomal events that are sensitive to DNA damage.

Purpose of the Study:

  • To evaluate the effects of bleomycin (BLM) on meiotic chromosomes in mice.
  • To investigate the impact of BLM on the synaptonemal complex (SC) during meiotic prophase.
  • To compare BLM's clastogenic activity during different stages of meiosis.

Main Methods:

  • Mice were treated with bleomycin (BLM) via intraperitoneal (i.p.) or intratesticular (i.t.) injection at various meiotic stages.

Related Experiment Videos

  • Chromosome aberrations were analyzed in meiotic metaphase I cells.
  • Synaptonemal complex (SC) integrity and synaptic errors were assessed in meiotic prophase cells (pachytene, leptotene-zygotene).

    • Main Results:

    • Intraperitoneal BLM injection during pre-meiotic S phase significantly increased structural damage in metaphase I cells.
    • Intratesticular and intraperitoneal BLM injections induced synaptic errors and SC anomalies in pachytene cells, similar to irradiation effects.
    • BLM treatment during meiotic prophase stages induced structural and synaptic anomalies, with some suggestion of interference with chiasma formation.

    Conclusions:

    • Bleomycin (BLM) is clastogenic to meiotic chromosomes in mice, with significant effects observed when administered during S phase.
    • BLM induces SC damage and synaptic errors during meiotic prophase, though structurally aberrant cells are not always recovered at metaphase I.
    • The study highlights germ-line specific responses to BLM's clastogenic activity, differing from its general meiotic activity.