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Updated: Jul 19, 2026

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
Published on: June 11, 2010
[Interspecies polymorphism of peroxidase genes, localized on chromosome 5 of Arabidopsis thaliana]
Abstract:
Comparative analysis of nucleotide sequences in five peroxidase genes AtPrx52-AtPrx56 located in the left arm of chromosome 5 was performed by using six Arabidopsis thaliana ecotypes and lines (Columbia, Dijon-M, Blanes-M, Enkheim-M, Ler, K-156). Significal differences (up to 20 times) in the levels of nucleotide variation between these genes were detected: tandem duplicated genes AtPrx53 and AtPrx54 have the highest and the AtPrx56 gene has the lowest level of nucleotide diversity. The genes AtPrx53 and AtPrx54 were characterized by allelic dimorphism; the nonrandom association between nucleotide polymorphic sites within the AtPrx54 was shown. The connection between gaplotype of these genes and the mobility of anionic peroxidase izoforms was detected. Since two gaplotypes of AtPrx53 were coding proteins, which differed by two significant amino acid substitutions, we supposed that differences in mobility of anionic peroxidase izoforms caused by the diallelic polymorphism in amino acid sequence of AtPrx53 protein.
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