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Cold acclimation and oxygen consumption in the thymus.

Clare M Brennan1, Eamon P Breen, Richard K Porter

  • 1School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland.

Biochimica Et Biophysica Acta
|October 7, 2006
PubMed
Summary
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Mitochondrial uncoupling protein 1 (UCP 1) was studied in rat thymus after cold acclimation. Despite increased UCP 1 expression, it does not play a thermogenic role in the thymus.

Area of Science:

  • Mitochondrial biology
  • Immunology
  • Physiology

Background:

  • Mitochondrial uncoupling protein 1 (UCP 1) is typically found in brown adipose tissue.
  • UCP 1 has recently been identified in rat and mouse thymus tissue.
  • The potential thermogenic function of UCP 1 in the thymus remains unexplored.

Purpose of the Study:

  • To investigate the thermogenic role of UCP 1 in rat thymus.
  • To examine the effects of cold acclimation on UCP 1 expression and function in the thymus.

Main Methods:

  • Rats were cold-acclimated for 5 weeks.
  • Assessed thymocyte oxygen consumption, mitochondrial abundance, and UCP 1 expression (immunoblotting, GDP binding).
  • Measured oxygen consumption rates in isolated thymus mitochondria.

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Main Results:

  • Cold-acclimated rat thymocytes showed significantly lower oxygen consumption rates and were insensitive to noradrenaline.
  • Thymus tissue and mitochondrial abundance did not increase with cold acclimation.
  • UCP 1 expression per unit of mitochondria increased (1.7-fold by immunoblotting, 1.5-fold by GDP binding).
  • Isolated thymus mitochondria exhibited increased state 4, GDP-inhibitable, and palmitate-activatable oxygen consumption.
  • Overall UCP 1 per gram of thymus tissue increased only 1.2-fold after cold acclimation.

Conclusions:

  • Despite increased UCP 1 expression and activity in thymus mitochondria, its overall contribution to thymus thermogenesis is minimal.
  • UCP 1 does not appear to have a significant thermogenic role in the rat thymus.