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Published on: September 19, 2017
Laccase immobilization in redox active layered double hydroxides: a reagentless amperometric biosensor
Christine Mousty1, Laetitia Vieille, Serge Cosnier
1Laboratoire d'Electrochimie Organique et de Photochimie Redox, UMR CNRS 5630, Institut de Chimie Moleculaire de Grenoble, FR CNRS 2607, Université Joseph Fourier, Grenoble, France. Christine.Mousty@ujf-grenoble.fr
A novel biosensor co-immobilizes laccase and a layered double hydroxide for sensitive dissolved oxygen detection. This system effectively identifies toxic anionic substances like azide, fluoride, and cyanide at low concentrations.
Area of Science:
- Electrochemistry
- Biosensors
- Environmental Science
Background:
- Enzyme inhibitors pose environmental and health risks.
- Accurate detection of dissolved oxygen is crucial for environmental monitoring.
- Biosensors offer sensitive and selective analytical platforms.
Purpose of the Study:
- To develop a new amperometric biosensor system.
- To detect dissolved oxygen and anionic toxic substances.
- To utilize co-immobilized laccase and layered double hydroxide for enhanced performance.
Main Methods:
- Co-immobilization of Trametes versicolor laccase and [Zn-Cr-ABTS] layered double hydroxide on a glassy carbon electrode.
- Amperometric detection based on the electrocatalytic reduction of oxygen (O2) at 0.2V using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as a mediator.
- Application of the biosensor for the determination of dissolved oxygen and specific anionic toxic substances.
Main Results:
- The biosensor demonstrated a fast and sensitive response for dissolved oxygen determination in the range of 6 x 10(-8) to 4 x 10(-6)M.
- Very low detection limits were achieved for azide (5.5 nM), fluoride (6.9 nM), and cyanide (6.2 nM).
- The system proved effective in detecting enzyme inhibitors, which are known anionic toxic substances.
Conclusions:
- The developed biosensor system is highly effective for the amperometric determination of dissolved oxygen.
- The biosensor shows significant potential for the sensitive detection of toxic anionic substances.
- Co-immobilization of laccase and layered double hydroxide provides a robust platform for electrochemical sensing applications.
