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Global and targeted quantitative proteomics for biomarker discovery.

Timothy D Veenstra1

  • 1SAIC-Frederick Inc., National Cancer Institute at Frederick, P.O. Box B, Frederick, MD 21702, United States. veenstra@ncifcrf.gov

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|October 7, 2006
PubMed
Summary

Advancements in proteomic technologies, particularly mass spectrometry (MS), enable biomarker discovery. This article reviews quantitative MS approaches for identifying diagnostic and therapeutic biomarkers by comparing healthy and diseased patient samples.

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Area of Science:

  • Proteomics
  • Biomarker Discovery
  • Mass Spectrometry

Background:

  • Recent advances in proteomic technologies facilitate biomarker discovery.
  • Mass spectrometry (MS) is a key technology for analyzing complex proteome samples.
  • Biomarker discovery studies compare protein abundance between healthy and diseased states.

Purpose of the Study:

  • To review quantitative mass spectrometry approaches for biomarker discovery.
  • To highlight the advantages and disadvantages of various quantitative methods.
  • To guide the identification of clinically useful biomarkers.

Main Methods:

  • Utilizing mass spectrometry (MS) for high-throughput data acquisition.
  • Comparing proteomic samples from healthy and diseased individuals.

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  • Employing various quantitative proteomic techniques.

    • Main Results:

    • Development of multiple quantitative approaches for comparing protein abundance.
    • Identification of differentially abundant proteins as potential biomarkers.
    • Evaluation of the suitability of different methods for clinical applications.

    Conclusions:

    • Quantitative mass spectrometry is crucial for biomarker discovery.
    • Understanding the strengths and weaknesses of different methods is essential for successful biomarker identification.
    • This review aids in selecting appropriate techniques for discovering clinically relevant biomarkers.