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Related Experiment Videos

High affinity insulin binding and insulin receptor-effector coupling: modulation by Ca2+.

P F Williams1, I D Caterson, G J Cooney

  • 1Department of Endocrinology, Royal Prince Alfred Hospital, Sydney, NSW, Australia.

Cell Calcium
|September 1, 1990
PubMed
Summary
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Calcium ions (Ca2+) and ruthenium red (RR) enhance insulin receptor binding and signaling in hepatoma cells. These findings suggest Ca2+ binding induces conformational changes, boosting insulin sensitivity and action.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Endocrinology

Background:

  • Insulin signaling is crucial for glucose and amino acid metabolism.
  • The insulin receptor's structure and function are key to its activity.
  • Calcium ions (Ca2+) role in insulin receptor modulation is not fully understood.

Purpose of the Study:

  • To investigate the effect of Ca2+ and ruthenium red (RR) on insulin binding and signaling.
  • To characterize the putative calcium binding site on the insulin receptor.
  • To understand how Ca2+ influences insulin receptor affinity and post-receptor events.

Main Methods:

  • Cultured HTC hepatoma cells were used for experiments.
  • Insulin binding assays were performed in the presence of Ca2+ and RR.

Related Experiment Videos

  • Insulin-stimulated amino acid and glucose uptake were measured.

    • Main Results:

    • Ca2+ and RR significantly increased high-affinity insulin binding.
    • These ions enhanced insulin sensitivity for amino acid and glucose uptake.
    • Receptor-effector coupling improved, reducing required receptor occupancy for maximal response.

    Conclusions:

    • Ca2+ and RR binding to the insulin receptor induces conformational changes.
    • These changes increase insulin receptor affinity and sensitivity.
    • Ca2+ likely acts on an early post-receptor event, enhancing insulin action.