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Cloning of a promoter used by sigma H RNA polymerase in Bacillus subtilis
H L Carter1, K M Tatti, C P Moran
1Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322.
Gene
|December 10, 1990
Abstract:
The secondary RNA polymerase sigma factor sigma H is essential for endospore development in Bacillus subtilis. However, only a few promoters that are used by RNA polymerase containing sigma H (E sigma H) have been identified. We used in vitro transcription of random cloned fragments of B. subtilis chromosomal DNA to identify a promoter that is used by E sigma H. This promoter is active before the onset of sporulation.