Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gluconeogenesis: re-evaluating the FOXO1-PGC-1alpha connection.

Marcia M Schilling1, James K Oeser, Jared N Boustead

  • 1Department of Molecular Physiology and Biophysics, Vanderbilt University Medical School, Nashville, Tennessee 37232, USA.

Nature
|October 7, 2006
PubMed
Summary

The transcription factor FOXO1 and co-activator PGC-1alpha do not directly interact to stimulate glucose-6-phosphatase (G6Pase) gene expression. This study reveals an indirect transcriptional relationship between FOXO1 and PGC-1alpha in regulating gluconeogenesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Morning glucagon disrupts insulin induced hepatic metabolic memory and subsequent afternoon glucose metabolism in canines.

Frontiers in endocrinology·2026
Same author

The role of glucose-6-phosphatase activity in glucose homeostasis and its potential for diabetes therapy.

Trends in molecular medicine·2024
Same author

Multiple promoter and enhancer differences likely contribute to augmented G6PC2 expression in human versus mouse pancreatic islet alpha cells.

Journal of molecular endocrinology·2024
Same author

Biochemical and metabolic characterization of a G6PC2 inhibitor.

Biochimie·2024
Same author

Integrative analysis of pathogenic variants in glucose-6-phosphatase based on an AlphaFold2 model.

PNAS nexus·2024
Same author

Identification of structural motifs critical for human G6PC2 function informed by sequence analysis and an AlphaFold2-predicted model.

Bioscience reports·2023

Area of Science:

  • Molecular Biology
  • Metabolic Regulation
  • Gene Expression Analysis

Background:

  • Increased glucose-6-phosphatase (G6Pase) expression in the liver elevates glucose production in diabetes.
  • Previous research suggested a direct synergistic interaction between transcription factor FOXO1 and co-activator PGC-1alpha in stimulating gluconeogenesis genes.

Discussion:

  • This study investigated the directness of the interaction between FOXO1 and PGC-1alpha on G6Pase gene expression.
  • Experimental results demonstrate that FOXO1 is not essential for PGC-1alpha-mediated stimulation of G6Pase.
  • Conversely, FOXO1 alone is insufficient to induce the observed stimulation.

Key Insights:

  • FOXO1 is neither required nor sufficient for PGC-1alpha to stimulate G6Pase-luciferase fusion gene expression.

Related Experiment Videos

  • The transcriptional synergy between FOXO1 and PGC-1alpha in regulating gluconeogenesis is mediated indirectly.
  • This finding challenges the proposed direct interaction model.

    • Outlook:

    • Further research is needed to elucidate the precise indirect mechanism linking FOXO1 and PGC-1alpha.
    • Understanding this indirect interaction could reveal new therapeutic targets for managing diabetes.
    • This work contributes to a deeper understanding of metabolic gene regulation.