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Updated: Jul 19, 2026

Lipid-Protein Membrane Structure-Function Characterization using Droplet Interface Bilayers
Published on: June 12, 2026
Planar bilayer studies reveal multiple conductance states for synthetic anion transporters
Riccardo Ferdani1, George W Gokel
1Department of Molecular Biology and Pharmacology, Washington University School of Medicine, Campus Box 8103, 660 S. Euclid Avenue, St. Louis, MO 63110, USA.
Abstract:
Compounds of the general type R(1)(2)NCOCH(2)OCH(2)CO-(Gly)(3)-Pro-(Gly)(3)-OCH(2)Ph insert in phospholipid bilayers and conduct ions. Different levels of activity were observed when R(1) was either decyl or octadecyl, as judged either by Cl(-) release, detected by ion selective electrodes, or carboxyfluorescein dequenching, detected by fluorescence. Either method reports average behavior for all ionophores over all liposomes. These methods also show that at least two ionophores are involved in the formation of each pore. Planar bilayer experiments reported here confirm pore formation by these compounds but identify more than one conductance state for each. The pseudo-dimer, in which two molecules of the type shown above are covalently linked, shows only two conductance states, of which one is dominant. This state has been characterized by use of a current-voltage plot.
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