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Updated: Jul 19, 2026

Evaluation of Blood Lactate and Plasma Insulin During High-intensity Exercise by Antecubital Vein Catheterization
Published on: May 18, 2018
Effects of acute carbohydrate supplementation during sessions of high-intensity intermittent exercise
Maysa Vieira de Sousa1, Herbert Gustavo Simões, Marie Oshiiwa
1Faculty of Pharmaceutical Sciences, Department of Food and Experimental Nutrition, University of São Paulo, São Paulo, SP, Brazil. maysavs@uol.com.br
Abstract:
The present study evaluated the acute effects of carbohydrate supplementation on heart rate (HR), rate of perceived exertion (RPE), metabolic and hormonal responses during and after sessions of high-intensity intermittent running exercise. Fifteen endurance runners (26 +/- 5 years, 64.5 +/- 4.9 kg) performed two sessions of intermittent exercise under carbohydrate (CHO) and placebo (PLA) ingestion. The sessions consisted of 12 x 800 m separated by intervals of 1 min 30 s at a mean velocity corresponding to the previously performed 3-km time trial. Both the CHO and PLA sessions were concluded within approximately 28 min. Blood glucose was significantly elevated in both sessions (123.9 +/- 13.2 mg dl(-1) on CHO and 147.2 +/- 16.3 mg dl(-1) on PLA) and mean blood lactate was significantly higher in the CHO (11.4 +/- 4.9 mmol l(-1)) than in the PLA condition (8.4 +/- 5.1 mmol l(-1)) (P < 0.05). The metabolic stress induced by the exercise model used was confirmed by the elevated HR (approximately 182 bpm) and RPE (approximately 18 on the 15-point Borg scale) for both conditions. No significant differences in plasma insulin, cortisol or free fatty acids were observed during exercise between the two trials. During the recovery period, free fatty acid and insulin concentrations were significantly lower in the CHO trial. Supplementation with CHO resulted in higher lactate associated with lipolytic suppression, but did not attenuate the cortisol, RPE or HR responses.
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