Related Experiment Video
Updated: Jul 19, 2026
05:51
A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia
Published on: June 15, 2011
Dog Y chromosomal DNA sequence: identification, sequencing and SNP discovery
Christian Natanaelsson1, Mattias C R Oskarsson, Helen Angleby
1School of Biotechnology, KTH, Royal Institute of Technology, AlbaNova University Center, 10691 Stockholm, Sweden. cnat@kth.se
BMC Genetics
|October 10, 2006
Summary
Researchers identified novel dog Y-chromosome sequences and genetic markers, providing the first insights into canine Y-chromosome phylogeny and male dog population history. This study advances canine genetics research.
Area of Science:
- Canine genetics
- Population genetics
- Molecular biology
Background:
- Previous canine population studies relied on mitochondrial DNA, offering insights only into female lineages.
- A need exists for Y-chromosome studies to understand male dog history and provide independent genetic markers.
- Limited dog Y-chromosome sequence data and lack of reported polymorphisms hindered research.
Purpose of the Study:
- To identify and characterize male-specific Y-chromosome sequences in dogs.
- To discover Y-chromosome polymorphisms for population genetic analyses.
- To establish the initial Y-chromosome phylogeny of domestic dogs.
Main Methods:
- Comparative genomics: analyzed male dog genome against human Y chromosome and female dog genome to identify candidate sequences.
- Polymerase Chain Reaction (PCR): tested male specificity of candidate sequences using male and female dog DNA.
- Sequencing: analyzed Y-chromosome sequences from a diverse global collection of male dogs.
Main Results:
- Identified 24,159 bp of male-specific dog Y-chromosome sequence.
- Discovered nine Y-chromosome haplotypes defined by 14 single nucleotide polymorphisms (SNPs) across 10 male dogs.
- Phylogenetic analysis suggested origins from at least five ancestral wolf haplotypes, with no clear geographic distribution pattern.
Conclusions:
- Successfully identified significant dog Y-chromosome sequence for population genetic studies.
- Established a foundation for future SNP-based analyses of canine Y-chromosome diversity.
- Provided the first description of domestic dog Y-chromosome phylogeny, enhancing our understanding of male canine lineage history.
Related Concept Videos
02:26Comparing Copy Number Variations and SNPs
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
01:57Sanger Sequencing
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
01:29Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
02:54Evolutionary Relationships through Genome Comparisons
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
03:00Next-generation Sequencing
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
01:05Single Nucleotide Polymorphisms-SNPs
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...