Jove
Visualize
Contact Us

Related Experiment Videos

Replicative helicases: a staircase with a twist.

Mark S Dillingham1

  • 1Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK. Mark.Dillingham@bristol.ac.uk

Current Biology : CB
|October 10, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopy.

Nucleic acids research·2026
Same author

Mechanism of DNA capture by the MukBEF SMC complex and its inhibition by a viral DNA mimic.

Cell·2025
Same author

Simulation of cell cycle effects on DNA strand break induction due to α-particles.

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2024
Same author

Recent insights into eukaryotic double-strand DNA break repair unveiled by single-molecule methods.

Trends in genetics : TIG·2023
Same author

Structures of RecBCD in complex with phage-encoded inhibitor proteins reveal distinctive strategies for evasion of a bacterial immunity hub.

eLife·2022
Same author

Long DNA constructs to study helicases and nucleic acid translocases using optical tweezers.

Methods in enzymology·2022
Same journal

A global response contributes to tissue size robustness upon local induction of apoptosis.

Current biology : CB·2026
Same journal

Prebilaterian origin of monoaminergic signaling.

Current biology : CB·2026
Same journal

CLASP-dependent microtubule stabilization generates microtubule-based protrusive forces during Drosophila epithelial morphogenesis.

Current biology : CB·2026
Same journal

Pigeons make slow, divergent eye movements during flight and large, convergent eye movements when landing.

Current biology : CB·2026
Same journal

Temperature signals drive grass secondary cell wall thickening.

Current biology : CB·2026
Same journal

Neuronal RNAi and oxygen-sensing circuit shape germline resilience to heat stress.

Current biology : CB·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

The first crystal structure of a ring helicase shows how it moves along single-stranded DNA. This reveals the mechanism for ATP-dependent DNA translocation by the enzyme.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Biology

Background:

  • Ring helicases are essential enzymes involved in DNA replication and repair.
  • Understanding their mechanism is crucial for deciphering DNA processing pathways.

Purpose of the Study:

  • To determine the first crystal structure of a ring helicase encircling single-stranded DNA.
  • To elucidate the mechanism of ATP-dependent DNA translocation.

Main Methods:

  • X-ray crystallography
  • Biochemical assays

Main Results:

  • The crystal structure reveals the ring helicase encircling single-stranded DNA.
  • The structure provides insights into the conformational changes associated with ATP binding and hydrolysis.

Related Experiment Videos

  • A mechanism for ATP-dependent DNA translocation is proposed.

    • Conclusions:

    • The determined structure provides a molecular basis for understanding ring helicase function.
    • This work sheds light on the fundamental process of DNA translocation.