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Effect of the major repeat sequence on mitotic recombination in Candida albicans

Paul R Lephart1, Paul T Magee

  • 1Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 01702, USA.

Genetics
|October 10, 2006
PubMed

Insights

The major repeat sequence (MRS) in Candida albicans does not increase mitotic recombination. Mitotic recombination occurs throughout the MRS and adjacent DNA, similar to rates in yeast.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • The major repeat sequence (MRS) influences karyotypic variation in Candida albicans.
  • MRS impacts chromosome instability through repeat dynamics, chromosome loss, and translocations.

Purpose of the Study:

  • To investigate the role of the MRS in mitotic recombination in Candida albicans.
  • To compare recombination frequencies within the MRS to adjacent genomic regions.

Main Methods:

  • Examined mitotic recombination frequency across an average-sized MRS (approx. 50 kb).
  • Assessed recombination rates in a 325-kb DNA segment adjacent to the MRS.
  • Conducted the first large-scale analysis of mitotic recombination in Candida albicans.

Main Results:

  • Mitotic recombination frequencies were not elevated within the MRS compared to the adjacent 325-kb region.
  • Recombination events were detected throughout both the MRS and the adjacent DNA.
  • This study represents the first large-scale analysis of mitotic recombination in Candida albicans.

Conclusions:

  • The MRS does not appear to enhance mitotic recombination in Candida albicans.
  • Mitotic recombination occurs broadly across chromosome 5, including within the MRS.
  • Recombination rates in Candida albicans are comparable to those observed in Saccharomyces cerevisiae.

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