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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
Acetylation is important for MyoD function in adult mice
Arnaud Duquet1, Anna Polesskaya, Sylvain Cuvellier
1Laboratoire Epigénétique et cancer, CNRS FRE 2944, Institut André Lwoff, 7 rue Guy Moquet, 94801 Villejuif, France.
EMBO Reports
|October 10, 2006
Summary
MyoD acetylation on lysines 99 and 102 is crucial for adult muscle regeneration. Disrupting this modification in mice impairs muscle repair and differentiation, highlighting its in vivo importance.
Area of Science:
- Molecular Biology
- Cell Biology
- Developmental Biology
Background:
- Acetylation is a key post-translational modification affecting protein activity.
- The myogenic transcription factor MyoD's activity increases in vitro when acetylated on lysines 99 and 102.
Purpose of the Study:
- To investigate the in vivo biological relevance of MyoD acetylation at lysines 99 and 102.
- To determine the functional impact of MyoD acetylation on myogenesis and muscle regeneration in live animals.
Main Methods:
- Utilized specific antibodies to detect endogenous MyoD acetylation in myoblasts.
- Generated knock-in mice with mutations replacing lysines 99 and 102 of MyoD with arginines (MyoD(R99,102)).
- Analyzed embryonic development, adult muscle regeneration in vivo, and myoblast differentiation in vitro.
Main Results:
- Endogenous MyoD is confirmed to be acetylated on lysines 99 and 102 in myoblasts.
- MyoD(R99,102) knock-in embryos developed normally, despite slightly reduced MyoD levels.
- Adult MyoD(R99,102) homozygous mice exhibited phenotypes similar to MyoD-knockout mice, including delayed muscle regeneration and impaired myoblast differentiation.
Conclusions:
- MyoD acetylation on lysines 99 and 102 is essential for adult myogenesis and effective muscle regeneration in vivo.
- This post-translational modification plays a critical role in regulating the differentiation potential of myoblasts.
- The study underscores the significance of MyoD acetylation for the functional integrity of adult muscle tissue.
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