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Updated: Jul 19, 2026
Breath Collection from Children for Disease Biomarker Discovery
Published on: February 14, 2019
Urinary F2-isoprostane metabolite levels in children with sleep-disordered breathing
Hawley E Montgomery-Downs1, Jyoti Krishna, L Jackson Roberts
1Department of Psychology, West Virginia University, Morgantown, WV 26506-6040, USA.
Insights
Oxidative stress, measured by F2-isoprostane metabolites (IsoP-m), was not elevated in children with sleep-disordered breathing. This suggests oxidant stress is not a significant factor in pediatric sleep-disordered breathing.
Area of Science:
- Pediatric Medicine
- Cardiovascular Health
- Sleep Science
Background:
- Oxidative stress mechanisms are implicated in cardiovascular issues associated with adult sleep-disordered breathing.
- Isoprostanes are established biomarkers for in vivo oxidant injury.
Purpose of the Study:
- To investigate if children with sleep-disordered breathing exhibit elevated urinary F2-isoprostane metabolites (IsoP-m) as an indicator of oxidative stress.
- To test the hypothesis linking oxidative stress to pediatric sleep-disordered breathing.
Main Methods:
- Urinary samples were collected from 47 pediatric patients upon waking after polysomnography.
- Levels of F2-isoprostane metabolites (IsoP-m) were measured in urine samples.
- Data were analyzed controlling for Body Mass Index (BMI) and oxygen saturation (SpO2).
Main Results:
- No significant increase in urinary IsoP-m levels was observed in children with sleep-disordered breathing (mild, moderate, or severe).
- IsoP-m values did not correlate with any polysomnographic measures after controlling for BMI and SpO2.
Conclusions:
- The findings indicate that urinary F2-isoprostane metabolites are not elevated in children experiencing sleep-disordered breathing.
- Oxidative stress does not appear to be a significant pathophysiological feature of pediatric sleep-disordered breathing based on this biomarker.
Abstract:
Oxidant stress-related mechanisms have been proposed as a major contributor to the increased prevalence of cardiovascular morbidity in adult patients with sleep-disordered breathing. Isoprostanes provide a reliable biomarker of oxidant injury in vivo. The purpose of the present study was to examine the hypothesis that oxidant stress, as evidenced by increased levels of F2-isoprostane metabolites (IsoP-m) in urine, is present in children with a spectrum of sleep-disordered breathing. Assays were performed on urinary samples obtained from each of 47 pediatric patients immediately upon awakening after standard overnight polysomnography. Of the subjects, 15% had mild, 9% had moderate, and 6% had severe sleep-disordered breathing. After controlling for correlations between BMI and IsoP-m and SpO2 values, IsoP-m values were unrelated to any polysomnographic measures. The absence of increased levels of urinary F2-isoprostane metabolites in children with sleep-disordered breathing suggests that oxidative stress is not a significant feature of pediatric sleep-disordered breathing.
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