Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two factor H-related proteins from the mouse: expression analysis and functional characterization.

Jens Hellwage1, Florian Eberle, Tanja Babuke

  • 1Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany.

Immunogenetics
|October 10, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The zone of calcified cartilage: A human explant culture model and a proof-of-concept transcriptomic study of 3D laser-cut interface tissue.

Osteoarthritis and cartilage·2026
Same author

A new perspective on AMD pathogenesis: a sequential Factor H-centered view of complement dysregulation.

Frontiers in immunology·2026
Same author

Nanotube vesicles: a new neutrophil inflammatory response.

Blood·2026
Same author

Integrated Histology and Molecular Profiling of Postmortem Human Auditory and Vestibular Organs via a Poly (Methyl Methacrylate)-Based Workflow.

Journal of the Association for Research in Otolaryngology : JARO·2026
Same author

Deletion of the Mouse Homolog of Human FHR1 (muFHR1) Alleviates Atherosclerosis in ApoE-/- mice.

International journal of medical sciences·2026
Same author

Combined FXIII-C3 autoantibodies elicit bleeding and complement dysfunction in autoimmune FXIII deficiency.

The Journal of clinical investigation·2025

Researchers identified two complement factor H-related (FHR) proteins in mice. They characterized one FHR protein, FHR-B, as a heparin- and C3b-binding protein, offering insights into its in vivo function.

Area of Science:

  • Immunology
  • Complement System Biology

Background:

  • Complement factor H-related (FHR) proteins share structural and functional similarities with factor H (FH).
  • FHRs are found across species, but murine FHRs remain largely uncharacterized.
  • Mice serve as a valuable model for studying FHRs in vivo.

Purpose of the Study:

  • To characterize murine complement factor H-related (FHR) proteins.
  • To identify and analyze the expression and function of FHR proteins in mice.

Main Methods:

  • Detection of FHR proteins in mouse plasma using FH-specific antiserum.
  • Identification of FHR-encoding transcripts from a mouse liver cDNA library.
  • Real-time polymerase chain reaction (PCR) for tissue expression analysis.
  • Expression and purification of recombinant FHR-B protein.

Related Experiment Videos

  • Ligand binding assays with heparin and human C3b.

    • Main Results:

    • Two putative FHR proteins (approx. 100 and 38 kDa) were detected in mouse plasma.
    • Three murine FHR transcripts were identified: FHR-C, FHR-C_v1, and FHR-B.
    • FHR-B, composed of five short consensus repeat (SCR) domains, showed homology to FH.
    • FHR-B transcripts were highly expressed in liver, kidney, and heart, with liver levels exceeding FH.
    • Recombinant FHR-B and native presumptive FHR proteins bound to heparin and human C3b.

    Conclusions:

    • Two distinct FHR proteins exist in mice.
    • Murine FHR-B is a heparin- and C3b-binding protein, providing the first characterization of its in vivo binding properties.