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Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
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Published on: March 28, 2011

AUTO3DEM--an automated and high throughput program for image reconstruction of icosahedral particles

Xiaodong Yan1, Robert S Sinkovits, Timothy S Baker

  • 1Department of Chemistry & Biochemistry, University of California, San Diego, La Jolla, CA 92093-0378, USA. xyan@ucsd.edu

Journal of Structural Biology
|October 13, 2006
PubMed

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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