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Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

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Published on: July 4, 2011

Development of an optode membrane for high pH values.

Afsaneh Safavi¹, Marzieh Sadeghi

¹Department of Chemistry, Faculty of Sciences, Shiraz University, Shiraz 71454, Iran. safavi@chem.susc.ac.ir

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy

|October 13, 2006

Summary

Aniline Blue immobilized on a triacetylcellulose membrane creates a novel optical pH sensor. This sensor offers reliable, reversible measurements for high pH values (8.8-13), showing high sensitivity and stability.

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Area of Science:

Analytical Chemistry
Materials Science
Chemical Sensors

Background:

Accurate pH monitoring is crucial across various scientific and industrial applications.
Existing sensors may face limitations in high pH environments or require complex calibration.
Development of robust and sensitive optical pH sensors is an ongoing research area.

Purpose of the Study:

To develop and characterize a novel optical pH sensor for accurate measurements in high pH ranges.
To immobilize Aniline Blue onto a triacetylcellulose membrane for enhanced sensor performance.
To evaluate the sensor's stability, reversibility, sensitivity, and interference resistance.

Main Methods:

Immobilization of Aniline Blue dye onto an optically transparent triacetylcellulose membrane.
Spectrophotometric analysis of the immobilized dye's response to varying pH levels (8.8-13).
Assessment of sensor performance metrics including relative standard deviation, equilibration time, stability, and reversibility.

Main Results:

The developed optical sensor demonstrated effective pH measurements in the range of 8.8-13.
High sensitivity and reversibility were observed, with relative standard deviations of 1.6% and 2% for specific pH ranges.
The sensor exhibited rapid equilibration, long-term stability, and freedom from interference by other cations.

Conclusions:

The Aniline Blue-impregnated triacetylcellulose membrane serves as a practical and effective optical pH sensor for alkaline conditions.
The sensor's ease of fabrication, robustness, and reliable performance make it suitable for repetitive pH monitoring.
This development offers a valuable tool for applications requiring precise pH measurements in challenging high pH environments.