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Updated: Jul 19, 2026

Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV
03:53

Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV

Published on: November 10, 2023

Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses

X Sherry Chi1, Fenglan Li, John S Tam

  • 1Department of Clinical Immunology and Virology, Applied Immunology and Microbiology Division, Wyeth Vaccines Research, Bldg 180, 401N. Middletown Rd., Pearl River, NY 10965, USA.

Journal of Virological Methods
|October 13, 2006
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...