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Updated: Jul 11, 2026

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
Published on: May 29, 2011
Paramagnetic fluorescence quenching in a model membrane: a consideration of lifetime and temperature
D A Johnson1, B Nguyen, A F Bohorquez
1Division of Biomedical Sciences, University of California, Riverside, CA 92521-0121, USA. david.johnson@ucr.edu
Abstract:
To expand our understanding of paramagnetic quenching in membranes, the relationship between fluorophore excited-state lifetime (tau), temperature, and the collisional quenching was studied. Specifically, the ability of tempo to quench the steady-state and time-resolved emission from five lipophilic fluorophores (diphenylhexatriene, perylene, phenanthrene, pyrene, and triphenylene) partitioned into egg phosphatidylcholine (EggPC) liposomes was examined. Also, the temperature dependence of spin-labeled androstane to quench the emission (steady-state and time-resolved) from perylene in EggPC liposomes was determined. Unexpectedly, in EggPC liposomes, the apparent quenching efficiency decreased with increasing tau until the effect leveled off above approximately 20 ns. Moreover, in EggPC liposomes, dynamic quenching decreased with increasing temperature. The results suggest that in membranes, paramagnetic quenching is more complex than generally recognized.
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