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Updated: Jul 19, 2026
Establishment of Patient-Derived Xenograft Mouse Model with Human Osteosarcoma Tissues
Published on: March 22, 2024
Human xenograft osteosarcoma models with spontaneous metastasis in mice: clinical relevance and applicability for
Crispin R Dass1, Eugene T H Ek, Peter F M Choong
1Department of Orthopaedics, University of Melbourne, St. Vincent's Hospital, Fitzroy, VIC 3065, Australia. crispin.dass@svhm.org.au
Abstract:
Osteosarcoma cells derived from patients have been isolated and subsequently cultured for the past 35 years. To date though, there have been no major breakthroughs in the development of a model for osteosarcoma that uses orthotopic implantation of human osteosarcoma cells and that closely emulates the clinical progression of this debilitating and fatal disease. Such a model is long overdue given the devastating demographics (second highest cause of cancer-related death in the paediatric age group) of the ailment and the lack of solid options for control, if not cure, for the disease, as it also is the most common primary tumour of bone. Only then can more robust R & D be undertaken in the search for efficacious anti-osteosarcoma agents. This review tackles this conundrum and lists the variety of models (that use human osteosarcoma cells) available and the types of studies performed with these.
Insights
Developing accurate osteosarcoma models is crucial for advancing research. This review examines existing models using patient-derived cells to improve understanding and treatment of this bone cancer.
Area of Science:
- Oncology
- Orthopedic Surgery
- Cancer Research
Background:
- Osteosarcoma is a primary bone tumor and a leading cause of cancer death in children and adolescents.
- Current research models often fail to accurately replicate the clinical progression of osteosarcoma.
- There is a critical need for improved preclinical models to facilitate the development of effective treatments.
Purpose of the Study:
- To review existing models for osteosarcoma research that utilize patient-derived cells.
- To assess the suitability of current models for emulating the clinical progression of osteosarcoma.
- To highlight the importance of robust models for advancing osteosarcoma research and drug development.
Main Methods:
- Systematic review of literature on osteosarcoma models.
- Analysis of studies employing orthotopic implantation of human osteosarcoma cells.
- Evaluation of model fidelity in mimicking disease progression and patient demographics.
Main Results:
- A significant gap exists in validated orthotopic models that closely mimic human osteosarcoma progression.
- Various models using patient-derived osteosarcoma cells have been developed, but their clinical relevance varies.
- The review categorizes available models and the types of research conducted with them.
Conclusions:
- Improved preclinical models are essential for accelerating the discovery of novel anti-osteosarcoma agents.
- Further development of patient-derived orthotopic models is needed to better predict treatment efficacy.
- Robust research and development (R&D) hinges on the availability of clinically relevant osteosarcoma models.
