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Isolation and Activation of Murine Lymphocytes
Published on: October 30, 2016
Isolation of monoclonal antibodies monospecific for bovine alpha-lactalbumin
K M Kuzmanoff1, J W Andresen, C W Beattie
1Specialized Center for Cancer Research and Education, University of Illinois School of Medicine, Chicago 60612.
Abstract:
Monoclonal antibodies were generated against purified bovine alpha-lactalbumin. Three antibodies reactive only to alpha-lactalbumin were selected. Two of the antibodies appeared to be species monospecific and bound with only bovine alpha-lactalbumin. The third monoclonal recognized both bovine and human alpha-lactalbumin with a 2:1 preference for bovine alpha-lactalbumin. Two of the monoclonals (48-60, 48-68) bound to different epitopes with high affinity (Ka = 8 x 10(11), 5.3 x 10(12) M-1). The third monoclonal (48-22), which was of isotype IgA, had an epitope similar to that of 48-60 but with an apparent affinity of approximately 4 x 10(12) M-1. These antibodies should prove extremely useful adjuncts in structural studies of bovine alpha-lactalbumin.
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