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Suppression of interferon-induced oligo-2',5'-adenylate synthetase induction in persistent infection
N Fujii1, K Kimura, T Murakami
1Department of Microbiology, Sapporo Medical College, Japan.
Abstract:
Persistent infections with several strains of mumps virus (strains Torii and Miyahara), measles virus (strains Edmonston, CAM-70, AIK-C and Schwarz) and subacute sclerosing panencephalitis (SSPE) virus (Hälle and Mantooth) were established in various cell lines (FL, KB, A549, SK-AS, 293, K562, Ramos and NC-37). Oligo-2',5'-adenylate synthetase activity was demonstrated to be only slightly induced by interferon in cytoplasmic and nuclear fractions of cell lines persistently infected with mumps virus. In these cells, resistance to vesicular stomatitis virus infection was not induced by interferon treatment. Treatment of the persistently infected cells with interferon for 10 and 24 h did not stimulate an increase in the amount of synthetase mRNA. In cells persistently infected with measles and SSPE viruses, reduced induction of the enzyme varied with host cell types. Induction of the enzyme was not found in K562, SK-AS and KB cells, but was recognized in NC-37 and FL cells.
Insights
Persistent viral infections, including mumps, measles, and SSPE, showed limited interferon response. Interferon treatment did not induce antiviral resistance or increase key enzyme mRNA in these persistently infected cells.
Area of Science:
- Virology
- Cell Biology
- Immunology
Background:
- Persistent viral infections can alter cellular responses to antiviral agents.
- Interferon-stimulated genes, like oligo-2',5'-adenylate synthetase, are crucial for antiviral defense.
- Understanding these altered responses is key to developing effective treatments.
Purpose of the Study:
- To investigate the cellular response to interferon in cells persistently infected with mumps, measles, and SSPE viruses.
- To determine if interferon treatment can induce antiviral resistance in these infected cell lines.
- To assess the impact of persistent viral infection on interferon-induced gene expression.
Main Methods:
- Establishing persistent infections with mumps, measles, and SSPE virus strains in various human cell lines.
- Measuring oligo-2',5'-adenylate synthetase activity in cytoplasmic and nuclear fractions.
- Assessing resistance to vesicular stomatitis virus infection after interferon treatment.
- Quantifying synthetase mRNA levels following interferon exposure.
Main Results:
- Oligo-2',5'-adenylate synthetase activity was only slightly induced by interferon in mumps virus-infected cells.
- Interferon treatment failed to induce resistance to vesicular stomatitis virus infection in these cells.
- Interferon did not increase synthetase mRNA levels in persistently infected cells.
- Interferon-induced enzyme activity varied significantly across cell types for measles and SSPE virus infections, with no induction in K562, SK-AS, and KB cells.
Conclusions:
- Persistent infections with mumps, measles, and SSPE viruses significantly impair the cellular interferon response.
- The induction of oligo-2',5'-adenylate synthetase and antiviral resistance is compromised in these persistently infected cells.
- Cell type-specific variations in interferon response highlight the complexity of host-pathogen interactions.