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Related Experiment Videos

[Mast cell].

K Tasaka1

  • 1Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.

Nihon Kyobu Shikkan Gakkai Zasshi
|October 1, 1990
PubMed
Summary

Neuropeptides and histone trigger histamine release from mast cells by disrupting cell membranes and releasing intracellular calcium. This process involves conformational changes in neuropeptides and subsequent mast cell activation, contributing to airway hyperreactivity.

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Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Airway hyperreactivity is linked to histamine release from mast cells.
  • Neuropeptides and basic proteins are implicated in this histamine release.

Purpose of the Study:

  • Investigate the mechanisms of histamine release induced by neuropeptides and histone.
  • Elucidate the role of intracellular calcium and cell membrane interactions.

Main Methods:

  • Isolated rat peritoneal mast cells were used to study histamine release.
  • Calcium-free medium experiments were conducted to assess calcium release.
  • Model membrane systems were employed to examine neuropeptide-phospholipid interactions.

Main Results:

  • Substance P, somatostatin, neurotensin, and histone induced histamine release.
  • Significant intracellular calcium release was observed.
  • Neuropeptides underwent conformational changes upon interacting with lipid bilayers, leading to membrane perturbation and increased permeability.
  • Increased intracellular calcium activated cytoskeletal elements, causing granule extrusion.

Conclusions:

  • Neuropeptides and histone directly induce histamine release from mast cells.
  • The mechanism involves intracellular calcium release and direct interaction with the cell membrane.
  • These findings provide insights into the development of airway hyperreactivity.

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