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Studies on three structurally related phenylenediamines with the mouse micronucleus assay system
L Soler-Niedziela1, X Shi, J Nath
1Division of Plant and Soil Sciences, West Virginia University, Morgantown.
Mutation Research
|January 1, 1991
Summary
4-chloro-o-phenylenediamine (COP) is genotoxic in vivo, significantly increasing micronucleated cells in mice. However, 4-nitro-o-phenylenediamine (NOP) and p-phenylenediamine dihydrochloride (PPD) showed no genotoxic effects in this mouse bone-marrow micronucleus assay.
Area of Science:
- Toxicology
- Genetics
- Chemical Safety
Background:
- Structurally related compounds 4-chloro-o-phenylenediamine (COP), 4-nitro-o-phenylenediamine (NOP), and p-phenylenediamine dihydrochloride (PPD) are common in dyes and inks.
- COP is a known carcinogen in rodents, while NOP and PPD show in vitro genotoxicity.
Purpose of the Study:
- To evaluate the in vivo genotoxicity of COP, NOP, and PPD using the mouse bone-marrow micronucleus assay.
Main Methods:
- CD-1 male mice were injected with varying doses of COP, NOP, or PPD.
- Animals were sacrificed at 24, 48, and 72 hours post-injection.
- Micronucleated-polychromatic erythrocytes (MPCE) were quantified in bone-marrow cells.
Main Results:
- COP induced a significant, dose-related increase in MPCE, with peak response at 24 hours.
- No significant increase in MPCE was observed for NOP or PPD at any sampling interval.
Conclusions:
- COP demonstrates in vivo genotoxicity in the mouse bone-marrow micronucleus assay.
- NOP and PPD did not exhibit genotoxic effects in this in vivo study, despite prior in vitro findings.