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Practical Considerations for the Design, Execution, and Interpretation of Studies Involving Whole-Bone Bending Tests of Rodent Bones
Published on: September 1, 2023
Relationship between bicarbonate retention and bone characteristics in broiler chickens
M A Leslie1, R A Coleman, S Moehn
1Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada T6G 2P5.
Poultry Science
|October 13, 2006
Summary
The bicarbonate retention factor (BRF) in broilers was determined using radiolabeled bicarbonate. This factor is crucial for accurately measuring amino acid oxidation in growing birds.
Area of Science:
- Animal Nutrition
- Biochemistry
- Physiology
Background:
- The indicator amino acid oxidation technique requires accurate bicarbonate retention factors (BRF) for reliable results.
- Developing this technique in broilers necessitates understanding BRF across different growth stages.
Purpose of the Study:
- To determine the bicarbonate retention factor (BRF) in broilers aged 7 to 42 days.
- To investigate the relationship between bone mineral density and BRF.
- To establish a predictive model for BRF based on body weight.
Main Methods:
- Broilers (7-42 days old) received radiolabeled sodium bicarbonate (NaH14CO3).
- Expired 14C was measured to determine BRF.
- Femur bone mineral density was assessed using quantitative computed tomography.
Main Results:
- BRF peaked at 28 days, coinciding with rapid growth and bone deposition.
- A significant correlation (r=0.50) was found between femur bone mineral density and BRF.
- A predictive equation relating BRF to body weight (BW) was developed (R2=0.79).
Conclusions:
- A constant BRF of 35.15% was observed for broilers between 1,900 and 2,700 g BW.
- The established BRF and predictive equation will enhance the accuracy of future indicator amino acid oxidation studies in broilers.
- This research provides essential data for optimizing broiler nutrition and growth studies.
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