Related Experiment Video
Updated: Jul 19, 2026

Tree Core Analysis with X-ray Computed Tomography
Published on: September 22, 2023
Cork Oak Trees (Quercus suber L.)
Rubén Alvarez1, Mariano Toribio, Millán Cortizo
1Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, C/. Catedrático Rodrigo Uría Sn, Oviedo, Spain.
Abstract:
A transformation system for selected mature Quercus suber L. trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries the nptII and uidA genes. Evidence of stable transgene integration is obtained by polymerase chain reaction for nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos are germinated and successfully transferred to soil.
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