Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cork Oak Trees (Quercus suber L.).

Rubén Alvarez1, Mariano Toribio, Millán Cortizo

  • 1Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, C/. Catedrático Rodrigo Uría Sn, Oviedo, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|October 13, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association of the Surgical Technique With the Structural Valve Deterioration of a Bioprosthesis: A Prospective Cohort Study.

Seminars in thoracic and cardiovascular surgery·2022
Same author

Hepatoduodenal fistula closure diagnosed and characterized Ecoendoscopically (EUS) and managed by OTSC CLIP OVESCO: A case report.

International journal of surgery case reports·2021
Same author

Digital Twin for Automatic Transportation in Industry 4.0.

Sensors (Basel, Switzerland)·2021
Same author

Hybrid Memetic Algorithm for the Node Location Problem in Local Positioning Systems.

Sensors (Basel, Switzerland)·2020
Same author

Local Wireless Sensor Networks Positioning Reliability Under Sensor Failure.

Sensors (Basel, Switzerland)·2020
Same author

Genetic Algorithm Approach to the 3D Node Localization in TDOA Systems.

Sensors (Basel, Switzerland)·2019
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

A new Agrobacterium tumefaciens transformation system enables genetic modification of cork oak (Quercus suber L.) trees. This breakthrough allows for stable transgene integration and the development of genetically modified cork oak plants.

Area of Science:

  • Plant Biotechnology
  • Forest Genetics
  • Molecular Biology

Background:

  • Cork oak (Quercus suber L.) is an economically important tree species.
  • Efficient genetic transformation methods are crucial for improving tree traits and understanding gene function.

Purpose of the Study:

  • Establish a reliable Agrobacterium tumefaciens-mediated transformation system for mature Quercus suber L. trees.
  • Confirm stable integration and expression of transgenes in cork oak.

Main Methods:

  • Utilized recurrently proliferating embryogenic masses from mature cork oak.
  • Inoculated embryos with Agrobacterium tumefaciens (strain AGL1) carrying the pBINUbiGUSint plasmid.
  • Confirmed transgene integration using Polymerase Chain Reaction (PCR) and Southern blotting.

Related Experiment Videos

  • Assessed transgene expression via the uidA gene (GUS assay).

    • Main Results:

    • Successfully established a transformation system for Quercus suber L.
    • Demonstrated stable integration of nptII and uidA transgenes.
    • Confirmed successful expression of the uidA gene in transgenic embryos.
    • Germinated transgenic embryos and successfully transferred them to soil, indicating plantlet viability.

    Conclusions:

    • The developed Agrobacterium tumefaciens system is effective for genetic transformation of Quercus suber L.
    • This provides a foundation for future genetic improvement and research in cork oak.
    • The successful regeneration of transgenic plants opens avenues for functional genomics and breeding.