Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Fruit Development, Structure, and Function

Fruit Development, Structure, and Function

Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.

Trihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...

Cerebellum: Anatomical Regions

Cerebellum: Anatomical Regions

The cerebellum, also known as the "little brain," is located in the posterior cranial fossa, inferior to the tentorium cerebelli and dorsal to the brainstem. It plays a significant role in motor control, coordination, and proprioception.
Cerebellar Structure
Externally, the cerebellum features a highly convoluted surface with numerous folia (narrow ridges) separated by shallow sulci (grooves). The cerebellum is divided into two hemispheres by a thin median structure known as the vermis. The...

The Pineal Gland

The Pineal Gland

The pineal gland, a diminutive endocrine structure named for its pinecone-shaped appearance, is situated atop the third ventricle within the diencephalon region of the forebrain. This gland, composed of secretory cells known as pinealocytes arranged in compact cords and clusters around dense particles of calcium salts, plays a pivotal role in hormonal regulation.
The primary secretion of the pineal gland is the hormone melatonin, derived from serotonin. The concentration of melatonin in the...

Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mathematical modeling of the immune response mediated by human T-helper lymphocytes in viral diseases.

Mathematical biosciences and engineering : MBE·2025

Same author

Microbiological and Physicochemical Approach in the Feeding of Superworm (<i>Zophobas morio</i>) with Petroleum-Derived Polymer Diets.

Microorganisms·2024

Same author

Effects of Songwriting Group Music Therapy Program among Informal Caregivers of Older Adults with Dependency.

Healthcare (Basel, Switzerland)·2024

Same author

Plant-growth-promoting bacteria from rhizosphere of Chilean common bean ecotype (<i>Phaseolus vulgaris</i> L.) supporting seed germination and growth against salinity stress.

Frontiers in plant science·2023

Same author

Validation of Reference Genes in a Population of Blueberry <i>(Vaccinium corymbosum)</i> Plants Regenerated in Colchicine.

Plants (Basel, Switzerland)·2022

Same author

Xeloda Oral, Trastuzumab, and Pertuzumab Combined Drug Therapy Reduced Cervical Lymphadenopathy and Dermal Involvement in Patient With Recurrent Breast Cancer: Case Report.

Journal of investigative medicine high impact case reports·2020

Search research articles

Related Experiment Video

Updated: Jul 19, 2026

Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis

Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis

Published on: June 30, 2023

Pineapple [Ananas comosus (L.) Merr].

Lourdes Yabor¹, Patricia Espinosa, Ariel D Arencibia

¹Bioplant Center, University of Ciego de Avila, Carretera a Morón km 9, CP 69450, Ciego de Avila, Cuba.

Methods in Molecular Biology (Clifton, N.J.)

|October 13, 2006

Summary

This study details a pineapple genetic transformation method using temporary immersion bioreactors (TIB) for efficient transgenic plant selection. The procedure optimizes callus, Agrobacterium interaction, and regeneration for mass production of non-chimera transgenic pineapples.

More Related Videos

Fruit Volatile Analysis Using an Electronic Nose

Fruit Volatile Analysis Using an Electronic Nose

Published on: March 30, 2012

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

Related Experiment Videos

Last Updated: Jul 19, 2026

Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis

Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis

Published on: June 30, 2023

Fruit Volatile Analysis Using an Electronic Nose

Fruit Volatile Analysis Using an Electronic Nose

Published on: March 30, 2012

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

Area of Science:

Plant Biotechnology
Molecular Biology
Agricultural Science

Background:

Developing efficient genetic transformation protocols for pineapple (Ananas comosus) is crucial for crop improvement.
Optimizing tissue culture conditions is essential for successful plant regeneration and genetic modification.

Purpose of the Study:

To describe a novel procedure for pineapple genetic transformation using temporary immersion bioreactors (TIB).
To optimize key tissue culture factors for enhanced Agrobacterium-mediated transformation and recovery of transgenic pineapple plants.

Main Methods:

Utilized regenerable pineapple callus as the starting material for Agrobacterium infection.
Employed filtered callus to create homogeneously sized cell clusters, improving contact with Agrobacterium tumefaciens.
Regenerated primary transgenic plants without initial selection pressure, enabling mass production.

Main Results:

Demonstrated the competence of pineapple callus cells for Agrobacterium infection.
Showcased how filtered callus enhances Agrobacterium virulence through increased cell contact and phenolic compound release.
Successfully produced a large number of putative transgenic pineapple plants.

Conclusions:

Temporary Immersion Bioreactor (TIB) technology is a powerful system for pineapple genetic transformation.
The described protocol facilitates the recovery of non-chimera transgenic pineapple plants via micropropagation with an appropriate selection agent.