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Bacterial Phylum Actinobacteria01:30

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Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
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Bacterial Phylum Planctomycetes

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Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device
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Actinomycetes (Streptomyces lividans).

Clarence I Kado1, Brian Kelly

  • 1Davis Crown Gall Group, University of California, Davis, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 13, 2006
PubMed
Summary

Agrobacterium tumefaciens can transfer its T-DNA into actinomycetes, not just plants. This bacterial transformation method enables genetic modification of antibiotic-producing Streptomyces.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Agrobacterium tumefaciens is recognized for plant transformation via T-DNA transfer.
  • Its capability to transfer T-DNA into fungi and actinomycetes is less explored.
  • Actinomycetes, including Streptomyces, are crucial antibiotic-producing soil organisms.

Purpose of the Study:

  • To describe procedures for transforming actinomycetes using Agrobacterium tumefaciens.
  • To leverage the promiscuous T-DNA transfer system for genetic manipulation of actinomycetes.
  • To explore the potential for generating novel mutants in antibiotic-producing bacteria.

Main Methods:

  • Utilizing the T-DNA transfer system of Agrobacterium tumefaciens.
  • Applying transformation procedures to members of the actinomycetes.

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  • Analyzing the integration of T-DNA derivatives into the Streptomyces chromosome.

    • Main Results:

    • Successful transformation of actinomycetes using Agrobacterium tumefaciens was achieved.
    • Integration of T-DNA derivatives into the Streptomyces chromosome was demonstrated.
    • This method offers a pathway for genetic modification of Streptomyces.

    Conclusions:

    • Agrobacterium tumefaciens provides a viable method for transforming actinomycetes.
    • T-DNA integration into the Streptomyces chromosome facilitates the generation of valuable mutants.
    • This technique expands the genetic engineering toolkit for antibiotic-producing bacteria.