Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Fermentation01:23

Microbial Fermentation

1.7K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.7K
Fungal Phylum Ascomycota01:28

Fungal Phylum Ascomycota

1.9K
Phylum Ascomycota, a major division within the subkingdom Dikarya, comprises a diverse range of fungal species, including both unicellular yeasts and filamentous molds such as Aspergillus and Penicillium. These fungi thrive in a variety of habitats, from aquatic ecosystems to terrestrial environments, playing crucial ecological and economic roles.Morphology and ReproductionThe defining characteristic of Ascomycetes, commonly referred to as sac fungi, is the ascus—a sac-like structure that...
1.9K
Fungal Group Zygomycota01:29

Fungal Group Zygomycota

1.7K
Zygomycota, previously classified as a distinct fungal group, are primarily terrestrial, saprophytic molds that play a crucial role as decomposers. Recent phylogenetic studies have revealed that these fungi are now divided into two major clades — Mucoromycota, which includes many symbiotic species, and Zoopagomycota, which primarily consists of parasitic and pathogenic fungi. These groups exhibit distinct ecological roles and reproductive strategies while sharing key structural and...
1.7K
Yeast Signaling01:28

Yeast Signaling

18.3K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
18.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TONSL suppresses polymerase theta-dependent tandem duplications through chromatin-guided repair.

Nature communications·2026
Same author

Site-saturation functional screens identify PALB2 missense variants associated with increased breast cancer risk.

Nature communications·2026
Same author

CFAP20 salvages arrested RNAPII from the path of co-directional replisomes.

Nature·2026
Same author

Division of labor within polymerase theta in repair of CRISPR-induced DNA breaks in <i>Arabidopsis thaliana</i>.

PNAS nexus·2025
Same author

Replication-IDentifier links epigenetic and metabolic pathways to the replication stress response.

Nature communications·2025
Same author

Crown Gall Induced by a Natural Isolate of <i>Brucella</i> (<i>Ochrobactrum</i>) <i>pseudogrignonense</i> Containing a Tumor-Inducing Plasmid.

Microorganisms·2025

Related Experiment Video

Updated: Mar 1, 2026

Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
07:38

Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism

Published on: September 30, 2018

44.1K

Yeast (Saccharomyces cerevisiae).

Paul J J Hooykaas1, Amke den Dulk-Ras, Paul Bundock

  • 1Institute of Biology, Department of Molecular and Developmental Genetics, Leiden University, Clusius Laboratory, Leiden, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|October 13, 2006
PubMed
Summary

Saccharomyces cerevisiae, a well-studied yeast, reveals genetic needs for Agrobacterium-mediated DNA transformation. This research clarified DNA integration pathways, aiding fungal transformation methods.

More Related Videos

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
08:54

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

Published on: September 17, 2016

10.8K
Dissection of Saccharomyces Cerevisiae Asci
12:57

Dissection of Saccharomyces Cerevisiae Asci

Published on: May 19, 2009

19.5K

Related Experiment Videos

Last Updated: Mar 1, 2026

Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
07:38

Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism

Published on: September 30, 2018

44.1K
Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
08:54

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

Published on: September 17, 2016

10.8K
Dissection of Saccharomyces Cerevisiae Asci
12:57

Dissection of Saccharomyces Cerevisiae Asci

Published on: May 19, 2009

19.5K

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • Saccharomyces cerevisiae is a highly characterized eukaryotic model organism.
  • Understanding DNA transformation is crucial for genetic studies and biotechnology.
  • Agrobacterium-mediated transformation provides a key method for introducing foreign DNA into eukaryotes.

Purpose of the Study:

  • To elucidate the genetic requirements for Agrobacterium-mediated DNA transformation in Saccharomyces cerevisiae.
  • To investigate the mechanisms of DNA integration into eukaryotic chromosomes.
  • To establish a foundation for developing transformation protocols in other yeast and fungal species.

Main Methods:

  • Utilizing Saccharomyces cerevisiae as a model system for transformation studies.
  • Employing Agrobacterium tumefaciens for DNA delivery.
  • Analyzing DNA integration events through recombination pathways.

Main Results:

  • Identified specific genetic factors influencing Agrobacterium-mediated DNA transformation efficiency.
  • Demonstrated that DNA integration occurs via homologous recombination (preferred in S. cerevisiae) or nonhomologous end-joining.
  • Established a robust protocol for yeast transformation.

Conclusions:

  • Saccharomyces cerevisiae serves as an effective model for dissecting DNA transformation processes.
  • The study clarified the dual pathways of DNA integration in eukaryotes.
  • The developed methodology facilitates broader applications in fungal genetics and biotechnology.