Related Experiment Video
Updated: Jun 30, 2026

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
Published on: September 24, 2015
Tagging of avidin immobilized beads with biotinylated YAG:Ce3+ nanocrystal phosphor
Ryo Asakura1, Tetsuhiko Isobe, Kiyoshi Kurokawa
1Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, 223-8522, Japan.
Abstract:
YAG:Ce3+ nanoparticles 9.5+/-1.2 nm in diameter have been synthesized from aluminium isopropoxide and acetates of yttrium and cerium in 1,4-butanediol (1,4-BD) by autoclave treatment at 300 degrees C for 2 h. After replacing 1,4-BD by ultrapure water, NH2 groups were introduced on the surface of YAG:Ce3+ nanoparticles by addition of 3-aminopropyltrimethoxysilane then biotinylation with sulfo-NHS-LC-biotin. We demonstrated that avidin immobilized beads are tagged by biotinylated YAG:Ce3+ nanoparticles by the selective avidin-biotin interaction, furnishing a green fluorescent image on excitation with blue light. This result indicates that YAG:Ce3+ nanoparticle phosphors have much potential in biological applications.
More Related Videos
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Tagging and Fusion Proteins
Immunogold Electron Microscopy

