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In vivo molecular probing of cellular compartments with gold nanoparticles and nanoaggregates

Janina Kneipp1, Harald Kneipp, Margaret McLaughlin

  • 1Wellman Center for Photomedicine, Harvard University, Medical School, Boston, Massachusetts 02114, USA. jkneipp@janina-kneipp.de

Nano Letters
|October 13, 2006
PubMed

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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.