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A simple and rapid method for the detection of RNA in formalin-fixed, paraffin-embedded tissues by PCR amplification
F von Weizsäcker1, S Labeit, H K Koch
1Massachusetts General Hospital, Harvard Medical School, Charlestown.
Biochemical and Biophysical Research Communications
|January 15, 1991
Abstract:
A simple and rapid method for the extraction, reverse transcription and PCR amplification of RNA from formalin-fixed, paraffin-embedded tissue is described. The procedure can be completed within 24 hours. In a first application of this method we detect human albumin mRNA in liver tissue, demonstrating the feasibility to retrospectively analyze gene expression and RNA viruses in fixed tissue.