Related Experiment Video
Updated: Jul 19, 2026

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
Published on: August 31, 2017
Genotyping of clinical herpes simplex virus type 1 isolates by use of restriction enzymes
Peter Norberg1, Tomas Bergström, Jan-Ake Liljeqvist
1Department of Clinical Virology, Göteborg University, Guldhedsgatan 10 B, S-413 46 Göteborg, Sweden. peter.norberg@microbio.gu.se
Abstract:
Recently, three distinct genotypes of clinical herpes simplex virus type 1 (HSV-1) isolates were identified based on DNA sequence information and phylogenetic analysis of clinical isolates and laboratory strains. We utilized single-nucleotide polymorphism within the genes coding for glycoproteins G and I for rapid genotype classification by PCR and restriction enzyme cleavage. The method is suitable for high-scale genotyping of clinical HSV-1 isolates and for the detection of recombinants.
More Related Videos
Related Concept Videos
Herpes
Genital Herpes
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Antiviral Nucleoside Inhibitors

